• Search for biological correlates of non-suicidal behavior

    Currently, practically nothing is known about the biological processes occurring in the brain in most mental pathologies. One such pathology is non-suicidal self-injury (NSSI). Researchers from the IHNA&NPh RAS and the Moscow Research and Clinical Center for Neuropsychiatry have outlined a new direction of research - the search for correlates of this condition with the composition of small extracellular vesicles (sEVs) in the blood. The results of a pilot study on this topic were recently published. The work assessed the quantitative characteristics of sEVs in the blood of patients with NSSI and compared the concentration and size of sEVs in patients with major depressive disorder with and without NSSI, as well as assessed the relationship between the sizes and concentrations of sEVs in the sample with such parameters as the severity of anxiety, depression and suicidal risk. The size and concentration of the isolated particles were assessed using dynamic light scattering and nanoparticle tracking analysis.

    Authors found that NSSP in people with major depressive disorder is associated with a more severe course of the disorder (greater severity of depression, situational and personal anxiety), as well as a higher risk of suicide. The study did not reveal differences in the quantitative characteristics of sEV in patients with a depressive episode with and without NSSI. The authors believe that future research should be aimed at investigating the structural differences and functional features of sEVs in NSSI.

  • Increased levels of small extracellular vesicles in the blood of patients with depression, epilepsy and epilepsy with depression

    A recently published article by researchers from the IHNA&NPh RAS and the Moscow Research and Clinical Center for Neuropsychiatry raises new and interesting questions in the study of small extracellular vesicles (sEVs) in human blood. Previously, the team, using four different methods, had shown significant increase in the concentration of sEVs in the blood of patients with depression compared to healthy volunteers, and the followed work was intended to clarify the source of these “extra” vesicles in depression. In addition, the authors compared sEVs content in the blood of patients with a wide range of pathologies, including epilepsy, epilepsy with depression, bipolar affective disorder with a current depressive episode, and psychogenic nonepileptic seizures with depression. Small EVs were isolated from patient serum using gel filtration or polyethylene glycol (PEG) precipitation, and both methods showed very similar results. It turned out that in patients with depression, epilepsy, or epilepsy with depression, the total level of mBB in the blood is increased by up to two times compared to healthy volunteers. The authors of the work were able to go further and isolate the fraction of sEVs of neuronal origin (in the blood this is approximately every one hundred and fiftieth vesicle) in these patients, but no difference in the concentration of neuronal sEVs was found between any groups. The question of where the “extra” sEVs in the patients’ blood came from remains open. The authors suggest that the source of these sEVs are immune cells. However, the authors did discover at least one new finding that sheds light on the biogenesis of sEVs. It turned out that sEVs in the serum of both patients and healthy volunteers contain various lysosomal enzymes - and this is a hint that the contents of sEVs may reflect the state of the intracellular endolysosomal system.

  • Mother's diet suppresses epilepsy and depression in offspring

    The mother's diet during the perinatal period is the most important early environmental factor that, through epigenetic modifications, can cause changes in gene expression and, as a consequence, the phenotype of the offspring. Employees of the Institute of Higher Inspectorate and Scientific Branch of the Russian Academy of Sciences and the Institute of Molecular Genetics of the Russian Academy of Sciences were the first to show that increased DNA methylation in the early stages of ontogenesis using a maternal diet enriched with methyl supplements (MAD) suppresses the manifestation of genetic absence epilepsy and comorbid depression in the offspring of WAG/Rij rats . The beneficial phenotypic effect of maternal MOD was accompanied by increased expression of pathogenetically significant genes - the hcn1 ion channel gene and the DNA methyltransferase 1 (dnmt1) gene in the somatosensory cortex and hippocampus, as well as the hcn1, dnmt1 and tyrosine hydroxylase (th) genes in the nucleus accumbens. The therapeutic effect of maternal MOD was similar to that of ethosuximide, the first-choice drug for the treatment of absence epilepsy. It is assumed that maternal MOD can serve as a new preventive therapeutic strategy for epigenetic correction of absence epilepsy and comorbid depression in offspring.

    Статья опубликована в январе 2023 года в журнале Diagnostics: