Laboratory of Molecular Neurobiology

The Laboratory of Molecular Neurobiology appeared in 2007 as a group for the study of molecular neurobiology inside the laboratory of cellular neuroscience training. But already in 2010, a separate laboratory was formed under the leadership of Sergey Vladimirovich Salozhin. At this time, the laboratory mastered the basic methods of creating lentiviral vectors and packaging of lentiviral particles, as well as the creation of adeno-associated viruses and the technology of their concentration. In 2015, the laboratory was headed by Alexey Petrovich Bolshakov, and now the work is carried out in several priority areas:

Research of the epigenetic regulation of the processes of memory formation and storage. -Research of neuroinflammation.
Studies of gene expression during plastic rearrangements in the brain are normal and in models of CNS pathologies.
Investigations of the role of non-coding RNA in the functioning of CNS cells.
Electrophysiological and behavioral studies of the processes occurring in the brain of experimental animals with the spreading depression (one of the pathophysiological mechanisms of migraine) and the epileptiform activity of various genesis.
Optimization of methods for assembling various viral constructions (lentiviruses, adeno-associated viruses), packaging of viruses for use in laboratory and interlaboratory projects.

To perform work on projects in the laboratory, a wide range of methods is actively used: standard molecular biological (PCR, real-time PCR, RNA and DNA isolation, electrophoresis, cloning, etc.), chromatin precipitation (ChIP-pcr), western blot; work with primary cultures of neurons and cell lines PC12, HEK293; work with bacterial strains (transformation, plasmid production, purification); assembly of viral constructs (lentiviruses, adeno-associated viruses) for overexpression of target products in vitro and in vivo; work with animals (injection of viruses into various areas of the brain, in vivo electrophysiology, behavioral testing of emotional and cognitive disorders in CNS pathology, neuropharmacological methods; experimental modeling of epilepsy); immunohistochemistry, immunocytochemistry.

Список сотрудников

Publication list

Papers

Gabova A.V., Sarkisova K.Y. Maternal MethylEnriched Diet Normalizes Characteristics of the Sleep–Wake Cycle and Sleep Spindles in Adult Offspring of WAG/Rij Rats with Genetic Absence Epilepsy. Journal of Evolutionary Biochemistry and Physiology. 2023. V. 109. N. 1. P. 75-93. DOI: 10.31857/S086981392301003X
Medvedeva T.M., Sysoeva O.V., Sysoev I.V., Vinogradova L.V. Intracortical functional connectivity dynamics induced by reflex seizures. Experimental Neurology. 2023. V. 368. P. 114480. DOI: 10.1016/j.expneurol.2023.114480
Sarkisova K.Y., Fedosova E.A., Shatskova A.B., Rudenok M.M., Stanishevskaya V.A., Slominsky P.A. Maternal Methyl-Enriched diet increases DNMT1, HCN1, and TH Gene Expression and Suppresses Absence Seizures and Comorbid Depression in Offspring of WAG/Rij Rats. Diagnostics. 2023. V. 13. N. 398. P. 1-17. DOI: 10.3390/diagnostics13030398
Sarkisova K.Y., Gabova A.V., Fedosova E.A., Shatskova A.B., Narkevich V.B., Kudrin V.S. Antidepressant and anxiolytic effects of L-methionine in the WAG/Rij rat model of depression comorbid with absence epilepsy. International Journal of Molecular Sciences. 2023. V. 24. N. 15. P. 12425. DOI: 10.3390/ijms241512425
Sushkova O.S., Morozov A.A., Kershner I.A., Khokhlova M.N., Gabova A.V., Karabanov A.V., Chigaleichick L.A., Illarioshkin S.N. Investigation of Phase Shifts Using AUC Diagrams: Application to Differential Diagnosis of Parkinson’s Disease and Essential Tremor. Sensors. 2023. V. 23. N. 3. P. 1531. DOI: 10.3390/s23031531
Sarkisova K.Y., Fedosova E.A., Shatskova A.B., Narkevich V.B., Kudrin V.S. Maternal Methyl-Enriched Diet Increases Dopaminergic Tone of the Mesolimbic Brain System in Adult Offspring of WAG/Rij Rats. Doklady Biological Sciences. 2022. V. 506. N. 1. P. 145-149. DOI: 10.1134/S001249662205012X
Smirnova G.R., Medvedeva T.M., Pavlova I.V., Vinogradova L.V. Region-Specific Vulnerability of the Amygdala to Injury-Induced Spreading Depolarization. Biomedicines. 2022. V. 10. N. 9. P. 2183. DOI: 10.3390/biomedicines10092183
Spivak Y.S., Dobryakova Y.V., Medvedeva T.M., Markevich V.A., Bolshakov A.P. Deep Brain Stimulation of the Medial Septal Area Can Modulate Gene Expression in the Hippocampus of Rats under Urethane Anesthesia. International Journal Of Molecular Sciences. 2022. V. 23. N. 11. P. 6034. DOI: 10.3390/ijms23116034
Volobueva Yu.E., Dobryakova Y.V., Bolshakov A.P., Markevich V.A. Protective Effect of Nerve Growth Factor in the Hippocampus against Disturbances in Synaptic Plasticity Caused by Cholinergic Deficit. Neurochemical Journal. 2022. V. 16. N. 2. P. 147-154. DOI: 10.1134/S1819712422020155
Karan A.A., Spivak Y.S., Gerasimov K.A., Suleymanova E.M., Volobueva M.N., Kvichanskiy A.A., Vinogradova L.V., Bolshakov A.P. CB2 receptors modulate seizure-induced expression of pro-inflammatory cytokines in the hippocampus but not neocortex. Molecular Neurobiology. 2021. DOI: 10.1007/s12035-021-02395-w
Kolesov D.V., Ivanova V.O., Sokolinskaya E.L., Kost L.A., Balaban P.M., Lukyanov K.A., Nikitin E.S., Bogdanov A.V. Impacts of OrX and cAMP-insensitive Orco to the insect olfactory heteromer activity. Molecular Biology Reports. 2021. V. 48. N. 5. P. 4549-4561. DOI: 10.1007/s11033-021-06480-0
Mikhailova M.M., Volobueva M.N., Panteleyev A.A. Mechanisms driving the initiation and direction of endothelial sprouting in organotypic co-culture of aorta and spinal cord tissues. Cell Biochemistry And Function. 2021. DOI: 10.1002/cbf.3634
Nikitin E.S., Vinogradova L.V. Potassium channels as prominent targets and tools for the treatment of epilepsy. Expert Opinion On Therapeutic Targets. 2021. V. 6. P. 1-13. DOI: 10.1080/14728222.2021.1908263
Stepanichev M.Y., Goryakina T., Manolova A., Lazareva N., Kvichanskii A., Tretyakova L., Volobueva M., Gulyaeva N. Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects. Brain Structure and Function. 2021. V. 226. N. 2. P. 563-574. DOI: 10.1007/s00429-020-02199-z
Bolshakov A.P., Stepanichev M.Y., Dobryakova Y.V., Spivak Y.S., Markevich V.A. Saporin from Saponaria officinalis as a Tool for Experimental Research, Modeling, and Therapy in Neuroscience. Toxins. 2020. V. 12. N. 9. P. 546. DOI: 10.3390/toxins12090546
Medvedeva T.M. Estimating complexity of spike-wave discharges with largest lyapunov exponent in computational models and experimental data. AIMS Biophysics. 2020. V. 7. N. 2. P. 65-75. DOI: 10.3934/biophy.2020006
Sarkisova K.Y., Gabova A.V., Fedosova E.A., Shatskova A.B. Gender-Dependent Effect of Maternal Methyl-Enriched Diet on the Expression of Genetic Absence Epilepsy and Comorbid Depression in Adult Offspring of WAG/Rij Rats. Doklady Biological Sciences. 2020. V. 494. N. 1. P. 244-247. DOI: 10.1134/S0012496620050075
Sushkova O.S., Morozov A.A., Kershner I.A., Petrova N.G., Gabova A.V., Chigaleichik L.A., Karabanov A. Investigation of distribution laws of the phase difference of the envelopes of electromyograms of antagonist muscles in Parkinson’s disease and essential tremor patients. RENSIT: Radioelectronics. Nanosystems. Information technologies. 2020. V. 12. N. 3. P. 415-428. DOI: 10.17725/rensit.2020.12.415
Vinogradova L.V., Rysakova M.P., Pavlova I.V. Small damage of brain parenchyma reliably triggers spreading depolarization. Neurological Research. 2020. V. 42. N. 1. P. 76-82. DOI: 10.1080/01616412.2019.1709745
Vinogradova L.V., Suleymanova E.M., Medvedeva T.M. Transient loss of interhemispheric functional connectivity following unilateral cortical spreading depression in awake rats. Cephalalgia. 2020. V. 8. P. 333102420970172. DOI: 10.1177/0333102420970172
Borodinova A.A., Kuznetsova M.A., Alekseeva V.S., Balaban P.M. Histone acetylation determines transcription of atypical protein kinases in rat neurons. Scientific Reports. 2019. V. 9. N. 1. P. 4332. DOI: 10.1038/s41598-019-40823-z
Kvichanskiy A.A., Volobueva M.N., Spivak Y.S., Tretyakova L.V., Gulyaeva N.V., Bolshakov A.P. Expression of mRNAs for IL-1β, IL-6, IL-10, TNFα, CX3CL1, and TGFβ1 Cytokines in the Brain Tissues: Assessment of Contribution of Blood Cells with and without Perfusion. Biochemistry (Moscow). 2019. V. 84. N. 8. P. 905-910. DOI: 10.1134/S0006297919080066
Suleymanova E.M., Borisova M.A., Vinogradova L.V. Early endocannabinoid system activation attenuates behavioral impairments induced by initial impact but does not prevent epileptogenesis in lithium-pilocarpine status epilepticus model. Epilepsy & Behavior. 2019. V. 92. P. 71-78. DOI: 10.1016/j.yebeh.2018.12.001
Sushkova O.S., Morozov A.A., Gabova A.V., Karabanov A.V., Chigaleychik L.A. Investigation of the 0.5-4 Hz low-frequency range in the wave train electrical activity of muscles in patients with Parkinson’s disease and essential tremor. RENSIT: Radioelectronics. Nanosystems. Information technologies. 2019. V. 11. N. 2. P. 225-236. DOI: 10.17725/rensit.2019.11.225
Gabova A.V., Sarkisova K.Y., Fedosova E.A., Shatskova A.B., Morozov A.A. Age-related changes of spike-wave discharges in WAG/Rij rats with genetic absence epilepsy. Russian Journal of Physiology. 2018. V. 104. N. 10. P. 1176-1189. DOI: 10.7868/S0869813918100052
Sushkova O.S., Morozov A.A., Gabova A.V., Karabanov A.V. Application of the method of analysis of burst-like electrical activity of the brain to identify EEG features of patients at the early stage of Parkinson's disease. Zhurnal nevrologii i psikhiatrii im. S.S. Korsakova.. 2018. V. 118. N. 10. P. 1176-1179. DOI: 10.7868/S0869813918100052
Dobryakova Y.V., Kasianov A., Zaichenko M.I., Stepanichev M.Y., Chesnokova E.A., Kolosov P.M., Markevich V.A., Bolshakov A.P. Intracerebroventricular administration of <sup>192</sup>IgG-saporin alters expression of microglia-associated genes in the dorsal but not ventral hippocampus. Frontiers in Molecular Neuroscience. 2018. V. 10. P. 429. DOI: 10.3389/fnmol.2017.00429
Sarkisova K.Y., Gabova A.V. Maternal care exerts disease-modifying effects on genetic absence epilepsy and comorbid depression. Genes, Brain and Behavior. 2018. V. 17. N. 7. e12477. DOI: 10.1111/gbb.12477
Shaydurov V.A., Kasianov A., Bolshakov A.P. Analysis of Housekeeping Genes for Accurate Normalization of qPCR Data During Early Postnatal Brain Development. Journal of Molecular Neuroscience. 2018. V. 64. N. 3. P. 431-439. DOI: 10.1007/s12031-018-1037-y
Vinogradova L.V. Initiation of spreading depression by synaptic and network hyperactivity: Insights into trigger mechanisms of migraine aura. Cephalalgia. 2018. V. 38. N. 6. P. 1177-1187. DOI: 10.1177/0333102417724151
Vinogradova L.V., Shatskova A.B. Localizing and lateralizing values of postictal behavioral impairments in epileptic rats. Epilepsy & Behavior. 2018. V. 87. P. 195-199. DOI: 10.1016/j.yebeh.2018.07.016
Borodinova A.A., Zuzina A.B., Balaban P.M. Role of atypical protein kinases in maintenance of long-term memory and synaptic plasticity. Biochemistry (Moscow). 2017. V. 82. N. 3. P. 243-256. DOI: 10.1134/S0006297917030026
Kvichansky A.A., Volobueva M.N., Manolova A.O., Bolshakov A.P., Gulyaeva N.V. Neonatal proinflammatory stress alters the expression of genes of corticosteroid receptors in the rat hippocampus: Septo-temporal differences. Neurochemical Journal. 2017. V. 11. N. 3. P. 255-258. DOI: 10.1134/S1819712417030059
Medvedeva T.M., Sysoeva O.V., Sysoev I.V. Modeling spike-wave discharges by a complex network of neuronal oscillators. Neural Networks. 2017. V. 98. P. 271-282. DOI: 10.1016/j.neunet.2017.12.002
Nikitin E.S., Bal N.V., Malyshev A.Y., Ierusalimsky V.N., Spivak Y.S., Balaban P.M., Volgushev M.A. Encoding of high frequencies improves with maturation of action potential generation in cultured neocortical neurons. Frontiers in Cellular Neuroscience. 2017. V. 11. P. 28. DOI: 10.3389/fncel.2017.00028
Rozov A.V., Valiullina F.F., Bolshakov A.P. Mechanisms of long-term plasticity of hippocampal GABAergic synapses. Biochemistry (Moscow). 2017. V. 82. N. 3. P. 257-263. DOI: 10.1134/S0006297917030038
Sarkisova K.Y., Gabova A.V., Kulikov M.A., Fedosova E.A., Shatskova A.B., Morosov A.A. Rearing by foster Wistar mother with high level of maternal care counteracts the development of genetic absence epilepsy and comorbid depression in WAG/Rij rats. Doklady Biological Sciences. 2017. V. 473. N. 1. P. 39-42. DOI: 10.1134/S0012496617020077
Vinogradova L.V. Audiogenic kindling and secondary subcortico-cortical epileptogenesis: Behavioral correlates and electrographic features. Epilepsy and Behavior. 2017. V. 71. P. 142-153. DOI: 10.1016/j.yebeh.2015.06.014
Borodinova A.A., Salozhin S.V. Diversity of proBDNF and mBDNF functions in the central nervous system. Zhurnal Vysshei Nervnoi Deyatelnosti Imeni I.P. Pavlova. 2016. V. 66. N. 1. P. 3-23. DOI: 10.7868/S0044467716010044
Borodinova A.A., Salozhin S.V. Diversity of proBDNF and mBDNF functions in the central nervous system. Zhurnal Vysshei Nervnoi Deyatelnosti Imeni I.P. Pavlova. 2016. V. 66. N. 1. P. 3-23. DOI: 10.7868/S0044467716010044
Midzyanovskaya I.S., Biryukova L.M., Shatskova A.B., van Luijtelaar G., Tuomisto L.M. H1 histamine receptor densities are increased in brain regions of rats with genetically generalized epilepsies. Epilepsy Research. 2016. V. 127. P. 135-140. DOI: 10.1016/j.eplepsyres.2016.08.029
Suleymanova E.M., Gulyaev M.V., Abbasova K.R. Structural alterations in the rat brain and behavioral impairment after status epilepticus: An MRI study. Neuroscience. 2016. V. 315. P. 79-90. DOI: 10.1016/j.neuroscience.2015.11.061
Suleymanova E.M., Shangaraeva V.A., VanRijn C.M., Vinogradova L.V. The cannabinoid receptor agonist WIN55.212 reduces consequences of status epilepticus in rats. Neuroscience. 2016. V. 334. P. 191-200. DOI: 10.1016/j.neuroscience.2016.08.004
Sysoeva M.V., Vinogradova L.V., Kuznetsova M.A., Sysoev I.V., VanRijn C.M. Changes in corticocortical and corticohippocampal network during absence seizures in WAG/Rij rats revealed with time varying Granger causality. Epilepsy and Behavior. 2016. V. 64. P. 44-50. DOI: 10.1016/j.yebeh.2016.08.009
Vinogradova L.V., Grinenko O.A. Ictal electrographic pattern of focal subcortical seizures induced by sound in rats. Brain Research. 2016. V. 1635. P. 161-168. DOI: 10.1016/j.brainres.2016.01.027
Volkova E.P., Rozov A.V., Nadareishvili G.G., Bolshakov A.P. Corticosterone Induces Rapid Increase in the Amplitude of Inhibitory Response in Hippocampal Synapses with Asynchronous GABA Release. Bulletin of Experimental Biology and Medicine. 2016. V. 160. N. 5. P. 628-631. DOI: 10.1007/s10517-016-3234-4
Ierusalimsky V.N., Borodinova A.A., Balaban P.M. Localization of the atypical protein kinase Cζ in the Nervous System of the terrestrial snail Helix. Neyrokhimiya. 2015. V. 32. N. 4. P. 295-301. DOI: 10.7868/S1027813315040093
Dolgikh D.A., Malyshev A.Y., Salozhin S.V., Nekrasova O.V., Petrovskaya L.E., Roshchin M.V., Borodinova A.A., Feldman T.B., Balaban P.M., Kirpichnikov M.P., Ostrovsky M.A. Anion-selective channelrhodopsin expressed in neuronal cell culture and in vivo in murine brain: Light-induced inhibition of generation of action potentials. Doklady Biochemistry and Biophysics. 2015. V. 465. N. 1. P. 424-427. DOI: 10.1134/S160767291506023X
Matlashov M.E., Bogdanova Y.A., Ermakova G.V., Mishina N.M., Ermakova Y.G., Nikitin E.S., Balaban P.M., Okabe S., Lukyanov S.A., Enikolopov G., Zaraisky A.G., Belousov V.V. Fluorescent ratiometric pH indicator SypHer2: Applications in neuroscience and regenerative biology. Biochimica et Biophysica Acta - General Subjects. 2015. V. 1850. N. 11. P. 2318-2328. DOI: 10.1016/j.bbagen.2015.08.002
Vinogradova L.V. Comparative potency of sensory-induced brainstem activation to trigger spreading depression and seizures in the cortex of awake rats: Implications for the pathophysiology of migraine aura. Cephalalgia. 2015. V. 35. N. 11. P. 979-986. DOI: 10.1177/0333102414565672